Metagenome sequencing to unveil the occurrence and distribution of antibiotic resistome and in a wastewater treatment plant.

The emergence and persistence of antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs) has aroused growing public concern for its risk to human health and ecological safety. Moreover, heavy metals concentrated in sewage and sludge could potentially favour co-selection of ARGs and heavy metal resistance genes (HMRGs). In this study, the profile and abundance of antibiotic and metal resistance genes in influent, sludge and effluent were characterized based on the Structured ARG Datebase (SARG) and Antibacterial Biocide and Metal Resistance Gene Datebase (BacMet) by metagenomic analysis. Sequences were aligning against the INTEGRALL, ISFinder, ICEberg and NCBI RefSeq databases to obtain the diversity and abundance of mobile genetic elements (MGEs, e.g.plasmid and transposon). Among them, 20 types of ARGs and 16 types of HMRG were detected in all samples, the influent metagenomes contained many more resistance genes (both ARGs and HMRGs) than the sludge and the influent sample, large reductions in the relatively abundance and diversity of ARG were achieved by biological treatment. ARGs and HMRGs cannot be completely eliminated during the oxidation ditch. A total of 32 species of the potential pathogens were detected, relative abundances of pathogens had no obvious changes. It is suggested that more specific treatments are required to limit their proliferation in the environment. This study can be helpful for further understanding the removal of antibiotic resistance genes in the sewage treatment process via metagenomic sequencing.

Ghrelin suppresses hypoxia/reoxygenation-induced H9C2 cell pyroptosis via NLRP3.

To study the influence of ghrelin on hypoxia/reoxygenation (H/R) induced H9C2 cell pyroptosis by regulating NLRP3. H9C2 cells were categorized into 3 distinct groups: the control group (referred to as Control), the hypoxia-exposed group (abbreviated as H), and the hypoxia/reoxygenation-exposed group (referred to as H/R). The expression of ghrelin and NLRP3 was determined. Ghrelin overexpression cell line was established to analyze its effects on cell viability, cell cycle and apoptosis. Simultaneously, the assessment of NLRP3 and Caspase-1 expression levels was conducted. To further inspect the effect of ghrelin on H/R treated H9C2 cells via NLRP3, the experimental setups were formulated as follows: control group (Control), H/R group (abbreviated as H/R), Ghrelin overexpression group (Ghrelin), ghrelin overexpression and NLRP3 overexpression group (Ghrelin + NLRP3), NLRP3 overexpression group (NLRP3), NLRP3 negative control group (NLRP3-NC). Experiments mentioned above were performed in each group. In comparison to control, H/R cells expressed significantly lower level of ghrelin, but higher level of NLRP3. Further, a noteworthy reduction in cell viability was evident within the H/R group, with much more cells in G0/G1 phase and less in S phase, and with elevated cell death rate and protein levels of NLRP3 and caspase-1 (P<0.05). Overexpression of ghrelin was capable of increasing cell viability, reducing G0/G1 cell number while increasing S phase cells. Ghrelin overexpression could suppress cell apoptosis and both NLRP3 and caspase-1 expressions. NLRP3 overexpression could diminish the beneficial impacts of ghrelin on H/R treated H9C2 cells. Ghrelin exhibited the capability to suppress H/R induced H9C2 cell pyroptosis through inhibition of NLRP3.

Step-feeding food waste fermentation liquid as supplementary carbon source for low C/N municipal wastewater treatment: Bench scale performance and response of microbial community.

Municipal wastewater treatment often lacks carbon source, while carbon-rich organics in food waste are deficiently utilized. In this study, the food waste fermentation liquid (FWFL) was step-fed into a bench-scale step-feed three-stage anoxic/aerobic system (SFTS-A/O), to investigate its performance in nutrients removal and the response of microbial community as a supplementary carbon source. The results showed that the total nitrogen (TN) removal rate increased by 21.8-109.3% after step-feeding FWFL. However, the biomass of the SFTS-A/O system was increased by 14.6% and 11.9% in the two phases of the experiment, respectively. Proteobacteria was found to be the dominant functional phyla induced by FWFL, and the increase of its abundance attributed to the enrichment of denitrifying bacteria and carbohydrate-metabolizing bacteria was responsible for the biomass increase. Azospira belonged to Proteobacteria phylum was the dominant denitrifying genera when step-fed with FWFL, its abundance was increased from 2.7% in series 1 (S1) to 18.6% in series 2 (S2) and became the keystone species in the microbial networks. Metagenomics analysis revealed that step-feeding FWFL enhanced the abundance of denitrification and carbohydrates-metabolism genes, which were encode mainly by Proteobacteria. This study constitutes a key step towards the application of FWFL as a supplementary carbon source for low C/N municipal wastewater treatment.

Effect of microbial network complexity and stability on nitrogen and sulfur pollutant removal during sediment remediation in rivers affected by combined sewer overflows.

Discovering the complexity and improving the stability of microbial networks in urban rivers affected by combined sewer overflows (CSOs) is essential for restoring the ecological functions of urban rivers, especially to improve their ability to resist CSO impacts. In this study, the effects of sediment remediation on the complexity and stability of microbial networks was investigated. The results revealed that the restored microbial community structure using different approaches in the river sediments differed significantly, and random matrix theory showed that sediment remediation significantly affected microbial networks and topological properties; the average path distance, average clustering coefficient, connectedness, and other network topological properties positively correlated with remediation time and weakened the small-world characteristics of the original microbial networks. Compared with other sediment remediation methods, regulating low dissolved oxygen (DO) shifts the microbial network module hubs from Actinobacteria and Bacteroidetes to Chloroflexi and Proteobacteria. This decreases the positive association of networks by 17%-18%, which intensifies the competitiveness among microorganisms, further weakening the influence and transmission of external pressure across the entire microbial network. Compared with that of the original sediment, the vulnerability of the restored network was reduced by more than 36%, while the compositional stability was improved by more than 12%, with reduced fluctuation in natural connectivity. This microbial network succession substantially increased the number of key enzyme-producing genes involved in nitrogen and sulfur metabolism, enhancing nitrification, denitrification, and assimilatory sulfate reduction, thereby increasing the removal rates of ammonia, nitrate, and acid volatile sulfide by 43.42%, 250.68% and 2.66%, respectively. This study comprehensively analyzed the succession patterns of microbial networks in urban rivers affected by CSOs before and after sediment remediation, which may provide a reference for reducing the impact of CSO pollution on urban rivers in the subsequent stages.

Biomanipulation as a strategy for minimizing ecological risks in river supplied with reclaimed water.

Reclaimed water is an effective method for addressing water pollution and shortages. However, its use may contribute to the collapse of receiving water (algal blooms and eutrophication) owing to its unique characteristics. A three-year biomanipulation project was conducted in Beijing to investigate the structural changes, stability, and potential risks to aquatic ecosystems associated with the reuse of reclaimed water in rivers. During the biomanipulation, the proportion of Cyanophyta in the community structure of phytoplankton density in river supplied with reclaimed water decreased, and the community composition shifted from Cyanophyta and Chlorophyta to Chlorophyta and Bacillariophyta. The biomanipulation project increased the number of zoobenthos and fish species and significantly increased fish density. Despite the significant difference in aquatic organisms community structure, diversity index and community stability of aquatic organisms remained stable during the biomanipulation. Our study provides a strategy for minimizing the hazards of reclaimed water through biomanipulation by reconstructing the community structure of reclaimed water, thereby making it safe for large-scale reuse in rivers.

Microbial mechanisms of refractory organics degradation in old landfill leachate by a combined process of UASB-A/O-USSB.

A combined process of anaerobic digestion (UASB), shortcut nitrification-denitrification (A/O), and semi-anoxic co-metabolism (operated by an up-flow semi-anoxic sludge bed; USSB) was constructed for the treatment of old landfill leachate (>10 years). The performance and mechanism of refractory organics degradation by the combined process (UASB-A/O-USSB) were investigated. The results showed that the semi-anoxic co-metabolism contributes 57 % of the totally degraded refractory organics. Specific microorganisms and their corresponding metabolic functions drive the degradation of refractory organics in each unit of the UASB-A/O-USSB process. In detail, organics with simple molecular structures were preferentially degraded by anaerobic digestion and shortcut denitrification, whereas those with complex structures were subsequently degraded in the oxic tanks and USSB reactor by shortcut nitrification and semi-anoxic co-metabolism. The structural equation model showed that the combined process of shortcut nitrification and semi-anoxic co-metabolism had a better effect on the degradation of recalcitrant organics than the single process. These findings provide information on how refractory organics are metabolically degraded in a combined process.

New insights into cyanobacterial blooms and the response of associated microbial communities in freshwater ecosystems.

Cyanobacterial blooms are important environmental problems in aquatic ecosystems. Researchers have found that cyanobacterial blooms cannot be completely prevented by controlling and/or eliminating pollutants (nutrients). Thus, more in-depth basic research on the mechanism of cyanobacterial blooms is urgently needed. Cyanobacteria, being primordial microorganisms, provide habitats and have various forms of interactions (reciprocity and competition) with microorganisms, thus having a significant impact on themselves. However, little is known about how environmental conditions and microbial communities in both water and sediment jointly affect cyanobacterial blooms or about the co-occurrence patterns and interactions of microbial communities. We investigated changes in environmental factors and microbial communities in water and sediment during different cyanobacterial blooms and revealed their interacting effects on cyanobacteria. Cyanobacteria had greater competitive and growth advantages than other microorganisms and had antagonistic and aggressive effects on them when resources (such as nutrients) were abundant. Furthermore, microbial networks from cyanobacterial degradation periods may be more complex and stable than those from bloom periods, with more positive links among the microbial networks, suggesting that microbial community structures strengthen interconnections with each other to degrade cyanobacteria. In addition, we found that sediment-enriched cyanobacteria play a key role in cyanobacterial blooms, and sediment microorganisms promote the nutrient release, further promoting cyanobacterial blooms in the water bodies. The study contributes to further our understanding of the mechanisms for cyanobacterial blooms and microbial community structural composition, co-occurrence patterns, and responses to cyanobacteria. These results can contribute to future management strategies for controlling cyanobacterial blooms in freshwater ecosystems.

[Antibiotic resistance genes in aerosols: a review].

Recently, drug resistance resulted from the extensive abuse and over-use of antibiotics has posed a great threat to human health. Scholars have conducted numerous studies on the impacts of antibiotic resistant bacteria and antibiotic resistance genes (ARGs) in different types of environments. Aerosol is not only a potential reservoir for ARGs, but also an important route for transmission of ARGs in the environment. However, a systematic summary of its sources, transmission, human exposure, and health risks is lacking. This review focused on four types of typical sites for aerosol research: human functional living places, farms, urban wastewater treatment plants, and hospitals. The sources, transmission routes, human exposure, and health risks of ARGs in the aerosol of these four typical sites were reviewed. This article also provides a reference for prevention and control of ARGs in aerosols.

Metagenomic analysis of microbial community structure and distribution of resistance genes in Daihai Lake, China.

The emergence of resistance genes is a global phenomenon that poses a significant threat to both animals and humans. Lakes are important reservoirs of genes that confer resistant to antibiotics and metals. In this study, we investigated the distribution and diversity of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) in the sediment of Daihai Lake using high-throughput sequencing and metagenomic analysis. The results indicated that all sampling sites had similar bacterial community structures, with Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes being the most abundant. A total of 16 ARG types containing 111 ARG subtypes were deposited in the sediment. Among the resistance genes to bacitracin, multidrug, macrolide-lincosamide-streptogramin (MLS), tetracycline, beta-lactam, and sulfonamide were the dominant ARG types, accounting for 89.9-94.3% of the total ARGs. Additionally, 15 MRG types consisting of 146 MRG subtypes were identified. In all samples, MRGs of the same type presented resistance to Pb, Ni, Hg, W, Zn, Ag, Cr, Fe, As, Cu, and multimetals. Overall, the distribution and diversity of antibiotic and metal resistance genes showed no significant differences in the samples. Plasmids (91.03-91.82%) were the most dominant mobile genetic elements in the sediments of Daihai Lake. Network analysis indicated that the target ARGs and MRGs were significantly positively correlated with the microorganisms. Potential hosts for various ARGs and MRGs include Proteobacteria, Euryarchaeota, Actinobacteria, Chloroflexi, and Bacteroidetes.

[Application of nanopore sequencing in environmental microbiology research].

The development of high-throughput sequencing techniques enabled a deeper and more comprehensive understanding of environmental microbiology. Specifically, the third-generation sequencing techniques represented by nanopore sequencing have greatly promoted the development of environmental microbiology research due to its advantages such as long sequencing reads, fast sequencing speed, real-time monitoring of sequencing data, and convenient machine carrying, as well as no GC bias and no PCR amplification requirement. This review briefly summarized the technical principle and characteristics of nanopore sequencing, followed by discussing the application of nanopore sequencing techniques in the amplicon sequencing, metagenome sequencing and whole genome sequencing of environmental microorganisms. The advantages and challenges of nanopore sequencing in the application of environmental microbiology research were also analyzed.

[Metagenomic Analysis of Resistance Genes in Membrane Cleaning Sludge].

Wastewater treatment plants (WWTPs) are considered important reservoirs of antibiotic resistance genes (ARGs) and function as the main sources of ARGs in the environment. Membrane bioreactors (MBRs) have been recognized as effective tools for removing ARGs in WWTPs.There are a large number of pathogens and resistance genes in colloids, particulate matter, suspended matter, and microbial metabolites in intercepted wastewater by MBR. However, the distribution characteristics of resistance genes in membrane cleaning sludge remains unclear. In this study, resistance genes of membrane cleaning sludge were analyzed using a metagenomic technique. The results showed that there were 39 phyla in the membrane cleaning sludge. Proteobacteria, Nitrospirae, and Actinobacteria were the dominant phyla. The dominant genera were Nitrospira, Pseudomonas, and Bradyrhizobium. The pathogens accounted for 10.54% of all bacteria in the sample, among which Pseudomonas had the highest abundance, accounting for 3.94%. A total of 17 types of antibiotic resistance genes and 16 types of metal resistance genes (MRGs) (15 types of single metal resistance genes and 1 types of multi-heavy metal resistance gene) were identified. Multidrug resistance genes had the highest abundance, accounting for 49.08%. Multi-heavy metal resistance genes were the most abundant, accounting for 34.58%. The copper resistance genes were the most abundant of the single metal resistance genes, accounting for 19.99%. The most important functional pathway of microbial community in the membrane cleaning sludge was metabolic related, and many genes identified were related to human diseases. The numbers of genes related to bacterial resistance and bacterial infectious diseases were the largest, accounting for 34.50% and 16.62%, respectively. These results indicate that there were abundant ARGs, MRGs, and pathogens in the membrane cleaning sludge, which has potential environmental health risks. It is necessary to strengthen the control of ARGs, MRGs, and pathogens in membrane cleaning sludge to provide guidance for selecting appropriate technologies for effectively removing ARGs, MRGs, and pathogens.

Bibliometric analysis of zerovalent iron particles research for environmental remediation from 2000 to 2019.

Zerovalent iron (ZVI) has been a major focus of research and has attracted great attention during the last 2 decades by international researchers because of its excellent pollutant removal performance and several other merits in environmental remediation. Based on Web of Science Core Collection data, we present a comprehensive bibliometric analysis of ZVI research from 2000 to 2019. We analyze 4472 publications assuming three stages of growth trend of annual publication totals. We find that "The Chemical Engineering Journal" has been the most productive journal; Noubactep C is identified as the most productive author; China has been the most active country in this field and the Chinese Academy of Science the most productive institution. The timeline of keywords shows seven distinct co-citation clusters. In addition, the top 38 keywords with strong citation bursts are also detected, suggesting that the innovation of green composite synthesis of ZVI and nanoscale ZVI and its efficient removal capacity might be the prevailing research directions in the future.

New insights into restoring microbial communities by side-stream supersaturated oxygenation to improve the resilience of rivers affected by combined sewer overflows.

Combined sewer overflows (CSOs) are a dominant contributor to urban river pollution. Therefore, reducing the environmental impacts of CSOs and improving the self-purification capacity of water bodies are essential. In this study, the side-stream supersaturation (SSS) oxygenation was applied to restore microbial function of rivers which are affected by CSOs to improve the self-purification capacity. The results showed that apart from the dissolved organic matter inputs from CSO event, the sediment had become an important contributor to pollution in the studied river. After the long-term (46 d) implementation of SSS oxygenation, dissolved oxygen and the oxidation-reduction potential of the river water increased by 98% and 238%, respectively, compared to emergency control measures implemented following individual CSO events. The NH3-N concentrations and the chemical oxygen demand also decreased by 20% and 45%, respectively. In addition, the occurrence of microbial functions related to information storage and processing, and cellular process and signaling, increased by 1.87% and 0.82% in response to SSS oxygenation, respectively, and the Shannon index of the sediment microbial community increased by more than 15%. The frequencies of genes related to nitrification and sulfur oxidation also increased by 20-450% and >50%, respectively. This research provides new insights into the ecological restoration of rivers affected by CSOs.

Spatiotemporal differences in phosphorus release potential of bloom-forming cyanobacteria in Lake Taihu.

The abnormal elevation of cyanobacterial density and total phosphorus concentration after the reduction of exogenous pollutants in Lake Taihu is still an open question. An in-situ light-dark bottle method was used to investigate the spatiotemporal differences of phosphorus release potential of bloom-forming cyanobacteria (BFC) in Lake Taihu. Generalized additive model analysis (GAM) of field data revealed that the phosphorus release potential of BFC increased with the upregulation of Chlorophyll a (Chl-a) content per cell, which was further validated by the laboratory experiment results. We deduced that the accumulation of Chl-a content per cell might be an essential index of high phosphorus release potential of BFC. The phosphorus release potential of BFC was much higher in summer and autumn than that in spring and winter, while the phosphorus absorption potential increased with the rising of temperature. The distinct physiological status of BFC at different seasons brought about their variation in phosphorus release potential. Additionally, high phosphorus release potential of BFC region mainly concentrated in the eastern and the central, northwest, western, and the south of Lake Taihu in spring, summer, autumn, and winter, respectively. Further studies showed that the spatial differences in phosphorus release potential of BFC were most probably due to the horizontal drift of BFC driven by the prevailing wind. Collectively, the synergism of BFC's physiological status and horizontal drift determined the spatiotemporal differences of phosphorus release potential of BFC in Lake Taihu. Moreover, apparent spatiotemporal differences in phosphorus release potential of BFC were essential factors that induced the distinct distribution of total phosphorus in Lake Taihu. This study provides insight for exploring the reason for the constant increase of total dissolved phosphorus concentration and cyanobacterial density in Lake Taihu for the past 5 years.

A novel of transforming wastewater pollution into resources for desertification control by sand-consolidating cyanobacteria, Scytonema javanicum.

Cultivation of desert cyanobacteria in wastewater can lead to the optimal redistribution of regional resources and is likely to solve two global problems, i.e., wastewater pollution and desertification. However, the potential of using wastewater instead of traditional artificial culture media to cultivate sand-consolidating cyanobacteria for desert management is not well understood. This study compares undistilled and distilled wastewater with an artificial culture medium (BG110) to explore the potential of wastewater as a replacement culture medium for Scytonema javanicum. The results show that the photosynthetic activity (Fv/Fm) of S. javanicum was inhibited in the undistilled wastewater and was lower than that in distilled water and the culture medium. The lowest Chl-a concentration and the highest concentration in BG110 were found in distilled wastewater. However, there was no difference in the biomass (dry weight) between the undistilled wastewater and BG110 at the end of the experiment. After long-term dry storage of the biomass collected after cultivation, there was no difference in the photosynthetic recovery between S. javanicum cultivated in undistilled wastewater and that cultivated in BG110. Accordingly, although wastewater depressed the Chl-a content, it did not affect the biomass accumulation and subsequent photosynthetic recovery after long-term storage. The results reveal the significant potential of cultivating sand-consolidating cyanobacterium in wastewater and using this technology as a new nutrient redistribution method in human settlements and desert areas.

Effects of ambient temperature on the redistribution efficiency of nutrients by desert cyanobacteria- Scytonema javanicum.

Cultures of Scytonema javanicum obtained from artificial medium are used to control desertification, and through the effective redistribution of nutrients, related environmental problems can be alleviated. Wastewater is considered to be a potential alternative medium for S. javanicum. However, the effect of temperature on the nutrient redistribution ability of S. javanicum cultured in wastewater has rarely been considered. Therefore, this study explores the effect of temperature on S. javanicum in wastewater. The results showed that a sufficient temperature increase (from 25 °C to 30 °C) increased the photosynthetic activity of photosynthetic system II (PSII), accelerated the accumulation rate of S. javanicum biomass, and improved the removal efficiency of nutrients in wastewater. However, an increasing temperature caused a decrease in the final accumulated biomass. When the temperature was above 35 °C, the ratio of the variable to maximal fluorescence (Fv/Fm) of S. javanicum decreased, thus, causing damage to PSII. The average Fv/Fm at 35 °C and 40 °C decreased by 10.49% and 72.37%, respectively, compared to that at 25 °C. By analysing the chlorophyll fluorescence induction kinetics (OJIP) curve after 30 days, the P phase at 30 °C increased by 15.47% relative to that at 25 °C, whereas that at 35 °C and 40 °C decreased by 45.54% and 86.37%, respectively. In particular, at 40 °C, the O-J-I-P phase transformed into the O-J (J = I = P) phase, which caused irreversible damage to the PSII of S. javanicum. Comprehensive scores were determined using the entropy weight method and revealed that 30 °C was the optimal temperature for the wastewater culture of S. javanicum. This temperature improved the biomass accumulation rate and wastewater transfer efficiency. These results provide a scientific basis for improving the efficiency of the coupling technology of wastewater treatment and desert algal cultivation.

[Temporal and Spatial Variation of Zooplankton Community Structure and Its Relationship with Environmental Factors in Dianshan Lake, Shanghai].

Dianshan Lake, a subtropical shallow lake, is the largest freshwater body located in Shanghai. To reveal the temporal and spatial variation of zooplankton community structure and its relationships with environmental factors, monthly data of zooplankton and phytoplankton content and associated physicochemical parameters for 2017 were analyzed using multivariate regression trees (MRT) and principal coordinates analysis (PCoA). The results indicated that there were significant seasonal differences in the community structure of zooplankton (P<0.05). However, spatial variations were significant only in spring and summer (P<0.05). The results indicated that water temperature (WT), chlorophyll-a (Chla), ammonia nitrogen, and cyanobacteria were the key driving factors in the observed spatial and temporal variations in the zooplankton community structure. The MRT analysis illustrated that zooplankton community structure varied strongly across four groups, including spring (13.07℃ ≤ WT<19.57℃), summer (WT ≥ 19.57℃, Chla ≥ 9.03 µg·L-1), autumn (WT ≥ 19.57℃, Chla<9.03 µg·L-1), and winter (WT<13.07℃). In addition, three distinct regions were identified by the cluster analysis. The MRT analysis illustrated that the zooplankton community structure was distinct between samples with relatively lower (<1.11 mg·L-1) and higher concentrations (≥ 1.11 mg·L-1) of ammonia nitrogen in spring. Furthermore, cyanobacteria were identified as a major stressor on zooplankton in summer. These observations further show that that zooplankton community structure in area I (with a cyanobacterial biomass of ≥ 2.58 mg·L-1) was significantly different from other regions (with a cyanobacterial biomass of <2.58 mg·L-1).

[Effects of Nutrient Addition on the Growth and Competition of Bloom Forming Cyanobacterium Chrysosporum ovalisporum: An In-situ Experiment].

An in-situ mesocosm experiment was conducted to study the growth dynamic of Chrysosporum ovalisporum and the other phytoplankton organisms under accelerated eutrophication conditions by using 39 buckets (100 L) in Lake Dishui, Shanghai. The results showed that the growth of both filamentous cyanobacteria (C. ovalisporum) and green algae were promoted with nutrient enrichment. The increase in the algal biomass rate in N plus P addition treatments was significantly higher than in treatments with N or P alone (P<0.05). Although the increasing biomass rate with P addition alone was higher than with N alone, there was no statistically significant difference (P>0.05). The relevant abundance of C. ovalisporum showed a significantly decreasing trend with N addition treatments and N plus P additions treatments (P<0.05), although it was slightly increased with the treatments with P alone (P>0.05). Nutrient addition could significantly improve the growth of small sized algae organisms (Chroococcus spp., Coelastrum spp., Chlorella spp., Tetraedron spp., and Scenedesmus spp.) rather than C. ovalisporum in all treatments (P<0.05). The small sized green algae overcoming C. ovalisporum indicated that small sized algae were more favored by hyper-eutrophicated, high water temperature and relatively undisturbed conditions. This is because small sized algal organisms have higher metabolic and growth rates compared to other sized algae, especially in stationary water regimens and high, light density conditions. We foresee that the small sized algae, Chlorophyte, dominating the small hyper-eutrophic aquatic system may be a potential succession pattern in the high water temperature seasons.